Magnetic separation and immunoassay of multi-antigen based on surface enhanced Raman spectroscopy.

نویسندگان

  • Shuai Chen
  • Yaxian Yuan
  • Jianlin Yao
  • Sanyang Han
  • Renao Gu
چکیده

A novel and highly sensitive immunoassay method based on surface enhanced Raman spectroscopy (SERS) and magnetic particles has been developed. This method exhibits great potential application in bio-separation and immunoassay.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Biosensing Based on Surface-Enhanced Raman Spectroscopy by Using Metal Nanoparticles

Surface-enhanced Raman spectroscopy (SERS) is a promising tool in the analytical science because it provides good selectivity and sensitivity without the labeling process required by fluorescence detection. This technique consists of locating the target analyte on nanometer range of roughed Au-nanoparticles. The presence of the metal nanoparticles provides a tremendous enhancement to the result...

متن کامل

Magnetic separation of clenbuterol based on competitive immunoassay and evaluation by surface-enhanced Raman spectroscopy

The elimination of b-agonist has attracted considerable interest due to its harmfulness to human health when it existed in pork. Here, a strategy based on immuno-magnetic nanoparticles has been successfully developed for the selective and successive magnetic separation of two kinds of b-agonists, clenbuterol (CL) and salbutamol (SAL). The calibration curve of competitive immunoassay was determi...

متن کامل

Molecular Diagnosis of Plasma Phenylalanine in Neonates with Phenylketonuria Disease Using Biological Sensors Based on Surface-Enhanced Raman Spectroscopy (SERS)

In this study, silver nanoparticles were chemically synthesized and deposited on glass substrates using a reducing agent of sucrose, at 50°C. Different characterizations including atomic force microscopy (AFM), field emission scanning electron microscopy (FESEM), and Raman spectroscopy were obtained to study silvery substrates. Then, the silvery substrates were used as the SERS substrates to de...

متن کامل

Improved molecular fingerprint analysis employing multi-branched gold nanoparticles in conjunction with surface-enhanced Raman scattering

Vibrational spectroscopy is a powerful analytical tool that assesses molecular properties based on spectroscopic signatures. In this study, the effect of gold nanoparticle morphology (spherical vs multi-branched) was assessed for the characterization of a Raman signal (ie, molecular fingerprint) that may be helpful for numerous medical applications. Multi-branched gold nanoparticles (MBAuNPs) w...

متن کامل

A paper-based surface-enhanced resonance Raman spectroscopic (SERRS) immunoassay using magnetic separation and enzyme-catalyzed reaction.

In this study, a novel paper-based SERRS immunoassay based on magnetic separation and alkaline phosphatase (ALP) enzyme catalyzed hydrolysis reaction was developed. By using modified antibodies conjugated to magnetic beads, capture of mouse IgG followed by addition of ALP-labeled antibodies would form a sandwich-like immunoconjugate. After magnetic separation, 5-bromo-4-chloro-3-indolyl phospha...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 47 14  شماره 

صفحات  -

تاریخ انتشار 2011